I’m going to weigh in here if I may. Sorry, I don’t frequent these boards much (at all), but I have a lot of experience with Oxford Nanopore sequencing and GPU compute. First a collection of resources that may be useful to you:
OK, so that’s an overview of some hopefully useful bits and bobs. I would now like to comment on a few things. Firstly, you really want to hold on to your fast5 files, doing so allows you to return to them again and again as models improve accuracy and allow the deteciton of additional base modifications. Real-time basecalling while sequencing is awesome, and something like an RTX3060 can keep up easily in high accuracy mode (HAC), even in super high accuracy (SUP) with some tweaking. But you will always want to go back and basecall again after the fact. The Jetson Xavier AGX will not keep up with HAC calling in real-time (we’ve tried), but in FAST mode it’s great, you can actually run 2x MinIONs. The Clara AGX could easily keep up, but it’s not widely available yet. While the A10 is an amazing card, I would advise to spend that amount of money on other things. Something like a 308Ti or a 3090 is more than enough for 95% of people (probably 99.9% of people to be honest).
Sorry for the long post, happy to comment more if useful.