Hi NICK_LIN,
There is a level shift on GEN1_I2C, did you measured the 1.8V and 3.3V power rails? And what’s the voltage of SDA/SCL on both sides?
Since only this board has such issue, it seems like a hardware failure, please remove all components on GEN1_I2C, just leave pull up resistors of 1.8V side to check the voltage, if it is abnormal still, that should be the TK1 chip GEN1_I2C pins issue.