Clara-parabricks 4.2.0-1: is rna_fq2bam not designed for fragment lengths > 100 ( --sjdbOverhang > 100)?

for fragment lengths about 151bp (–sjdbOverhang 150) I guess greater than 100 (the default value) the rna_fq2bam is failing. It only runs when the genome index with compatible STAR version is created with --sjdbOverhang 100 (default value) but very slow. Is rna_fq2bam not expected to run for --sjdbOverhang > 100?

for genome index created with proper parameter choice --sjdbOverhang=(fragment length -1) i.e. 150. I keep getting error when running rna_fq2bam even though STAR version 2.7.2a was used to create genome index.

EXITING because of FATAL ERROR: Genome version: 20201 is INCOMPATIBLE with running STAR version: 2.7.2a
SOLUTION: please re-generate genome from scratch with running version of STAR, or with version: 2.7.1a

It runs is genome index is created with --sjdbOverhang=100 (only change) but very slow.

singularity exec --nv clara-parabricks_4.2.0-1.sif /bin/bash -c "nvidia-smi;
pbrun rna_fq2bam
--in-fq R1_001.fastq.gz R2_001.fastq.gz
--genome-lib-dir /genome_index/GRCh38_r110_bp151/STAR_2_7_2a/
--ref /genome_index//Homo_sapiens.GRCh38.110.dna_sm.primary_assembly.fa
--output-dir /Aligned/10206-TH-0001_S1_L005_GPU_basic_R/
--out-bam 10206-TH-0001_S1_L005_GPU_basic_R_Aligned.sortedByCoord.out.bam
--read-files-command zcat
--sjdb-overhang=150
--verbose
--x3" |& tee /Logs/10206-TH-0001_S1_L005_GPU_basic_R_GnomeIndex_STAR_2_7_2a_2024-01-29.log

Three genome indexes tried:
• Worked with
–sjdb-overhang=150
○ /bulk_RNAseq_gpu/genome_index/genomeParameters.txt
### /softwares/Programs/STAR-2.7.2a/bin/Linux_x86_64/STAR --runMode genomeGenerate --runThreadN 16 --genomeDir /bulk_RNAseq_gpu/genome_index/ --genomeFastaFiles /reference_files/Homo_sapiens.GRCh38.110.dna_sm.primary_assembly.fa --sjdbGTFfile /reference_files/Homo_sapiens.GRCh38.110.gtf --sjdbOverhang 100

• Failed: with --sjdb-overhang=150
	○ /genome_index/GRCh38_r110_bp151/
	○ /genome_index/GRCh38_r110_bp151/genomeParameters.txt
	### STAR   --runMode genomeGenerate   --runThreadN 16   --genomeDir /nobackup/genome_index/GRCh38_r110_bp151/   --genomeFastaFiles /genome_index//Homo_sapiens.GRCh38.110.dna_sm.primary_assembly.fa      --sjdbGTFfile /genome_index//Homo_sapiens.GRCh38.110.gtf   --sjdbOverhang 150
	
• Failed: with --sjdb-overhang=150
	○ /genome_index/GRCh38_r110_bp151/STAR_2_7_2a/
	○ /genome_index/GRCh38_r110_bp151/STAR_2_7_2a/genomeParameters.txt
	### STAR   --runMode genomeGenerate   --runThreadN 16   --genomeDir /genome_index/GRCh38_r110_bp151/STAR_2_7_2a/   --genomeFastaFiles /genome_index//Homo_sapiens.GRCh38.110.dna_sm.primary_assembly.fa      --sjdbGTFfile /genome_index//Homo_sapiens.GRCh38.110.gtf   --sjdbOverhang 150