I had the data and clock lines length match in the layout, verify the change from 3.3V to 1.8V and located the SD card connector as close as possible to the TX1 connector.
right now i don’t have too many options left.
my thoughts are that the traces of 4.3 mils are too thin, or that there is some interference from a clock signal on the board.
but i did manage to use 256GB microSD cards with no problem.
i there a way to get the TX1 to sweep the frequencies and write/read speed and output a report ?
i assume that clock speed is different for different card volume.
the other option is a driver issue, but on the evaluation board is works with the full size sd card adapter for the same microSD cards i tested.