SPI-app.cMISO and MOSI on Header 40 Pin connector?

Robotics & Edge Computing Jetson & Embedded Systems Jetson AGX Orin
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Hi philippe12,

First, I would suggest updating to the latest JP5.1.2(R35.4.1).

Yes, you could short PIN19 (SPI1_MOSI) and PIN21(SPI1_MISO) on 40 pins header.

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You could also refer to the following thread to verify SPI loopback test.
Jetson Nano SPI Bus Not Working - #10 by KevinFFF
Although it is on Jetson Nano, it should be similar steps for AGX Orin devkit.