Upgrading Mac Pro (2009) from to NVIDIA Quadro 4000, what PCI slot to use ?

I am sorry if this is the wrong forum but I did not find the right place to ask questions about the Quadro 4000 for Mac.

I am upgrading my Mac Pro (2009) from the Nvidia GT120 (stock) to the Nvidia Quadro 4000 for Mac. The GT120 was installed in the PCI Slot #1 and I would like to install the Nvidia Quadro 4000 in the PCI Slot #2, that way the Fan in the Graphics Card (Quadro 4000) will have better air circulation, is there any limitation or recommendation regarding the PCI slot to use ?, thanks a lot