It failed when run bamsort.
The cmd used:
pbrun bamsort --ref fix_WYDT04_HiFiasm_genome.fa --in-bam DY90_rmdup.bam --out-bam output.bam --sort-order coordinate
PS: haplotypecaller
, deepvariant
, rna_gatk
, etc. can be run correctly.
Thanks for any advice.