The last occurrence of isolcpus on the kernel command line would be the one which the system uses. In the case of “isolcpus=”, and no other content, then this is likely the same as deactivating the isolcpus previous content, and should do the job.
The trick here is that you have to look at what the kernel command line actually shows after boot, and not the config file which probably works. What do you see now from: cat /proc/cmdline