Hi Parabricks Team,
I’ve successfully used apptainer + pb to execute pb_run fastq2bam . It created my bam files and now I want to use pb_run haplotypecaller. But I get the following error:
[PB Error 2025-Sep-05 18:17:13][src/haplotype_vc.cpp:843] Number of GPUs requested (2) is more than number of GPUs (0) in the system., exiting.
my comand was:
nvidia-smi 1>&2
pbrun haplotypecaller \
--num-gpus 2 \
--ref Homo_sapiens_assembly38.fasta \
--in-bam "name.cram" \
--gvcf \
--out-variants "name.g.vcf.gz" \
--tmp-dir TMP \
--logfile name.hc.log \
the stderr is:
INFO: underlay of /etc/localtime required more than 50 (79) bind mounts
INFO: underlay of /usr/bin/nvidia-smi required more than 50 (374) bind mounts
Fri Sep 5 18:17:12 2025
+-----------------------------------------------------------------------------------------+
| NVIDIA-SMI 575.51.03 Driver Version: 575.51.03 CUDA Version: 12.9 |
|-----------------------------------------+------------------------+----------------------+
| GPU Name Persistence-M | Bus-Id Disp.A | Volatile Uncorr. ECC |
| Fan Temp Perf Pwr:Usage/Cap | Memory-Usage | GPU-Util Compute M. |
| | | MIG M. |
|=========================================+========================+======================|
| 0 NVIDIA A100-PCIE-40GB On | 00000000:21:00.0 Off | 0 |
| N/A 30C P0 33W / 250W | 0MiB / 40960MiB | 0% Default |
| | | Disabled |
+-----------------------------------------+------------------------+----------------------+
| 1 NVIDIA A100-PCIE-40GB On | 00000000:81:00.0 Off | 0 |
| N/A 30C P0 33W / 250W | 0MiB / 40960MiB | 0% Default |
| | | Disabled |
+-----------------------------------------+------------------------+----------------------+
+-----------------------------------------------------------------------------------------+
| Processes: |
| GPU GI CI PID Type Process name GPU Memory |
| ID ID Usage |
|=========================================================================================|
| No running processes found |
+-----------------------------------------------------------------------------------------+
[PB Info 2025-Sep-05 18:17:13] ------------------------------------------------------------------------------
[PB Info 2025-Sep-05 18:17:13] || Parabricks accelerated Genomics Pipeline ||
[PB Info 2025-Sep-05 18:17:13] || Version 4.5.0-1 ||
[PB Info 2025-Sep-05 18:17:13] || GPU-GATK4 HaplotypeCaller ||
[PB Info 2025-Sep-05 18:17:13] ------------------------------------------------------------------------------
[PB Error 2025-Sep-05 18:17:13][src/haplotype_vc.cpp:843] Number of GPUs requested (2) is more than number of GPUs (0) in the system., exiting.
I don’t know much about working with GPUs. Can you please tell me what I’m doing wrong ?
thanks !
P.
PS: I saw Error with clara-parabricks:4.0.1-1 fq2bam - Bad argument value: Number of GPUs requested is more than number of gpus in system but it doesn’t help