Jetson TX2 installed JetPack 3.0 USB Can not work

Jetson TX2 installed JetPack 3.0 The system can work but USB and mouse plug can not be used normally, can not work

What is your carriers board? Is is the default devboard by nvidia or a custom carriers board?
Have you tried both USB ports including the small size one?
lsusb command output?

Something to consider is that if you are using GUI mode, then the XInput driver is used on top of the other USB/mouse driver chain. So it might not even be USB which is failing. If certain parts of X11 are updated and an invalid XInput version is selected, then the mouse/keyboard will fail in GUI mode. You might check if this shows errors:

sha1sum -c /etc/nv_tegra_release

If that is error free, then you might check lsusb mentioned by @Andrey1984. Additionally, you could monitor dmesg while inserting or removing the USB connector for mouse/keyboard/HUB:

dmesg --follow

If USB is functioning correctly, and the sha1sum was error-free, you would want to post the content of “/var/log/Xorg.0.log”.